En el estudio original de Wuhan, publicado en Nature en Febrero:
A new cobi19 associated with human respiratory disease in China
Describe explícitamente lo siguiente:
"To investigate the possible aetiological agents associated with this disease, we collected bronchoalveolar lavage fluid (BALF) and performed deep meta-transcriptomic sequencing. The clinical specimen was handled in a biosafety level 3 laboratory at Shanghai Public Health Clinical Center. Total RNA was extracted from 200 μl of BALF and a meta-transcriptomic library was constructed for pair-end (150-bp reads) sequencing using an Illumina MiniSeq as previously described
4,
6,
7,
8. In total, we generated 56,565,928 sequence reads that were de novo-assembled and screened for potential aetiological agents. Of the 384,096 contigs assembled by Megahit
9, the longest (30,474 nucleotides (nt)) had a high abundance and was closely related to a bat SARS-like cobi19 (CoV) isolate—bat SL-CoVZC45 (GenBank accession number MG772933)—that had previously been sampled in China, with a nucleotide identity of 89.1% (Supplementary Tables
1,
2). The genome sequence of this bichito, as well as its termini, were determined and confirmed by reverse-transcription PCR (RT–PCR)
10 and 5′/3′ rapid amplification of cDNA ends (RACE), respectively. This bichito strain was designated as WH-Human 1 cobi19 (WHCV) (and has also been referred to as ‘2019-nCoV’) and its whole genome sequence (29,903 nt) has been assigned GenBank accession number MN908947."
No aislaron nada, hicieron un estudio metagenómico, y de esa "sopa genética" empezaron a secuenciar a saco, y
de los millones de secuencias que obtuvieron se quedaron una de 30,474 nucleótidos que se parecía a un cobi19 de murciélago.
Después dice que la secuencia final del bichito (29,903 nucleótidos) la obtuvieron por PCR a partir de eso, y la publicaron. NI SIQUIERA EXPLICAN LOS DETALLES DEL SECUENCIAMIENTO POR PCR (RT-PCR) DE LA "SECUENCIA FINAL" DE 29,903 NUCLEÓTIDOS. Se limita a referenciar un documento del 2015.
NO DICE NADA DE AISLAMIENTO PREVIO DEL bichito, nada de nada de nada...
En este estudio, el original, el primigenio, del que parten los demás,
no se aisló ningún bichito antes de proceder a secuenciarlo.
