Veamos todo lo que te comes, manipulador:
1- determinación de que la causa no es ninguna conocida:
Preliminary aetiological investigations excluded the presence of influenza bichito,
Chlamydia pneumoniae and
Mycoplasma pneumoniae using commercial pathogen antigen-detection kits, and this was confirmed by PCR. Other common respiratory pathogens, including human adenoviruses, also tested negative by quantitative PCR (qPCR) (Extended Data Fig.
2). Although a combination of antibiotic, antiviral and glucocorticoid therapy was administered, the patient exhibited respiratory failure and was given high-flow non-invasive ventilation. The condition of the patient did not improve after 3 days of treatment and he was admitted to the intensive care unit
2- Ahora viene la identificación de la cadena que no correspondía a nada conocido en humanos, que es lo que has puesto.
3- LUEGO comprobaron su similitud con un cobi19 de murciélago:
the longest (30,474 nucleotides (nt)) had a high abundance and was closely related to a bat SARS-like cobi19 (CoV) isolate—bat SL-CoVZC45
4- Lo siguiente fue estudiar la posible evolución de este cobi19 para determinar si era posible que proviniese del murciélago:
To determine the evolutionary relationships between WHCV and previously identified coronaviruses, we estimated phylogenetic trees on the basis of the nucleotide sequences of the whole-genome sequence, the non-structural protein genes
ORF1a and
ORF1b, and the main structural proteins encoded by the
S,
E,
M and
N genes (Fig.
2 and Extended Data Fig.
5). In all phylogenies, WHCV clustered with members of the subgenus Sarbecovirus, including the SARS-CoV that was responsible for the global SARS pandemic
1,
2 of 2002–2003, as well as a number of SARS-like coronaviruses that have been obtained from bats
5,
11,
12,
13. However, WHCV changed topological position within the subgenus Sarbecovirus depending on which gene was used, which suggests that recombination has occurred in this group of viruses in the past
5- Ahora toca el estudio de la estructura de las proteínas:
To better understand the potential of WHCV to infect humans, the receptor-binding domain (RBD) of its spike protein was compared with those of SARS-CoVs and bat SARS-like CoVs. The RBD sequences of WHCV were more closely related to those of SARS-CoVs (73.8–74.9% amino acid identity) and SARS-like CoVs, including strains Rs4874, Rs7327 and Rs4231 (75.9–76.9% amino acid identity), that are able to use the human ACE2 receptor for cell entry
11 (Supplementary Table
7). In addition, the RBD of the spike protein from WHCV was only one amino acid longer than the RBD of the spike protein from SARS-CoV (y sigue)
Al final vienen especificados todos los métodos seguidos en estos pasos.
Ahora, seguid con vuestra tontería de que no se ha identificado el bichito.
Más que nada porque luego, en base a esto, se ha encontrado en otros enfermos con los que se han hecho aislados, como los que he puesto. O sea, que aprendáis a leer y a NO manipular.
